Nanoparticles are applied for enrichment of target cells from complex matrix and thereby improve the efficiency of detection techniques. In an earlier study from our group the efficacy of a glycine coated magnetic nanoparticle to entrap standard strains of mastitis causing bacteria from milk samples and used it to identify the sensitivity of antibiotics for clinical application (Viswanathan et al., 2014). We have determined the efficacy of the particle in trapping the clinical isolates and also the sensitivity of PCR in detecting the multitude of pathogens and some of their resistance genes in a single tube assay. The nanoparticle not only enabled sensitive detection, but also enabled identification of drug resistant bacterial strains like Methicillin Resistant Staphylococcus aureus (MRSA).
In recent years, the egg and egg product contaminations, associated out breaks particularly by bacterial species were reported worldwide. In developing countries, reusable egg trays are used without recycling for storing and selling eggs. Several studies have reported a higher incidence of bacterial contamination occurring through the egg trays in retail outlets than in the eggs themselves. The shelf life of the egg is directly influenced by egg shell's quality.
The success of the research work including the fabrication of the egg tray, studies their anti-microbial activity, and their applications. To develop the egg tray, a hybrid mixture was prepared based on chitosan and gelatin. Later the silver nanoparticles were embedded on it. The above hybrid mix was coated on paper through manual spray coating. This hybrid mixture coated egg trays were used to store table and fresh farm eggs.Performance indicator:
Newcastle disease virus (NDV) is an avian virus that causes disease in more than 250 avian species. In the poultry industry, it causes major economic losses.
The success of the research work including the fabrication of the NDV conjugated nanoparticles, studies their vaccine delivery efficiency, and the immunological responses. To develop the Nano NDV, the CaP NPs was prepared based on -Cyclodextrin and calcium chloride. Later the nano particles were functionalized with APTES. The amine functionalized nanoparticles were mixed with NDV virus and delivered through oculonasal on chicken.Performance indicator:
In the milk industries, the instruments used for the milk sample cooling, mixing, transporting, bottling and packing were cleaned twice in a week with H2O2 as a contaminant. The H2O2 is also used as stabilizer in milk, in well-defined concentrations, such as the preparations of hard cheeses as an alternative to pasteurization.
In Human, the H2O2 is generated by various oxidase-catalyzed reactions; a highly sensitive determination method of H2O2 is applicable to measurements of low levels of various oxidases and their substrates such as glucose, lactate, glutamate, urate, xanthine, choline, cholesterol and NADPH. More ever the consumer product, such as the hair dyes contained large amount of hydrogen peroxide. So the sensitive detection of hydrogen peroxide (H2O2) plays an important role.
The success of the research work including the fabrication of the fluorescent nanoprobe, studies their specificity, sensitivity, stability and their applications. To develop the nanosenosors, a fluorescent nanoparticles was prepared based on rhodamineisocyanide and calcium phosphate. Later the Rho/CaP nanoparticles were functionalized with HRP. The functionalized nanoparticles were incubated with H2O2 in the presence of OPD. The end product was read at three different methods such as fluorometeric, colorimetric and visual detections.Analytical advantages of this kit:
Probiotic organisms are friendly organisms that can inhibit the pathogenic organisms by competitive inhibition, maintaining lower pH, production of bacteriocins etc., and has immunomodulation property. Probiotics are being used in various veterinary and human applications. TRPVB has undertaken development of Probiotic spray for dogs to maintain healthy oral cavity and skin.
Twenty two Lactic Acid Bacteria were isolated from oral/skin/ aural samples of healthy pups. They are identified up to species level by 16S rRNA sequencing and biochemical tests. The isolates were checked for their Probiotic characters by checking the attachment to dog buccal epithelial cells, antimicrobial activity against Staphylococci, E. coli, Hydrogen peroxide release, Antibiotic sensitivity etc. Depending on these characters 5 isolates namely Lactobacillus plantarum, Weissellacibari, Pediococcuspentosaceus, Enterococcus munditii, Enterococcus faecium were selected. The isolates were scaled up using fermenters and spray dried at Microbax India Ltd., Hyderabad. A probiotic formulation using consortium of these five organisms with varied counts per dose was formulated. Stability studies for spray dried powders indicated that when stored in 40⁰C survival is 100% till 12 months. After reconstitution, L. plantarum and W. cibaria were maintained for 30 days at 40⁰C whereas the Enterococcus/ Pediococcus were maintained for 60 days.
Clinical trials in dogs were conducted to check the efficacy and dose response of the formulation. Results of the study indicated that the spray is effective in reducing the halitosis, dental tartar, reducing CFU of aerobic and anaerobic bacteria by effective colonization of LAB. Daily spray for 4 days and once in 5 days for 4 times is effective for the above parameters of study. The effect was maintained till the study period, i.e 30 days after withdrawal of the spray.Objectives
Bovine tuberculosis (BTB) is a major animal health problem and the approved BTB diagnostic tests are single intradermal tuberculin test (SITT) using purified protein derivative (PPD) of bovine tuberculin and/or in vitro assays to measure BTB specific interferon gamma (IFN-γ).
TRPVB offers E.coli expressed TB specific Fusion protein of ESAT-6: CFP-10 (~30kDa) for research and diagnostic purposes.Application
The recent reports on the presence of extraneous viruses such as porcine circovirus and porcine parvovirus contamination in rotavirus vaccine and the identification of nodovirus in the insect cells which was used to produce human papilloma virus (HPV) vaccine had cautioned the regulators and vaccine industry about the need for extensive characterization of the vaccine banks and substrates.
The major sources of extraneous Viruses contamination in vaccine and cell banks are animal derived raw materials such as porcine trypsin, bovine serum, cell line, source of Virus isolation, etc used during the vaccine development and production process.
Currently the 9CFR 113 extraneous Virus testing protocol was practiced by vaccine industry to screen their Virus and cell banks. However, there is an extensive list of bovine, porcine and human Viruses which are not detected by laborious and time-consuming 9CFR 113 protocol.
Regulators and manufacturers are in great need of additional testing procedures which may be useful as adjuncts and replacements for the 9 CFR tests in quality control labs of vaccine manufacturers.Technology available:
The Tuberculosis (TB) Antibody Rapid Detection kit is a qualitative, single use and two-step immuno chromatographic screening test for the detection of antibodies to Mycobacterium tuberculosis complex in serum, whole blood and plasma from wild animals. It is intended to be used as a preliminary screening test and as an aid in the diagnosis of infection with tuberculosis in wild animals. Any reactive specimen with the Tuberculosis Antibody Detection Test must be confirmed with alternative testing method(s).Download Wild TB Alert Kit Brochure